Atamt1 1

Author: nqai

August undefined, 2024

WebJul 10, 2006 · When expressed in oocytes, AtAMT1;1 generated significant NH 4 + and CH 3 NH 3 + currents at 100 μM substrate, while K + was not transported by AtAMT1;1 (Fig. 1 A). In water-injected oocytes, no significant currents were detected, as described previously [12].External pH had little effect on NH 4 + transport by AtAMT1;1 in oocytes (Fig. 1 B), … WebThe NH 4 1 concentration needed to saturate AtAMT1;2 was much higher than for AtAMT1;1, which has a more than 10-fold higher affinity (Mayer and Ludewig, 2006; Wood et al., 2006).

(PDF) Genome-wide identification and expression analysis

WebAnnotation: AMT1;1 locus:2140877 AT4G13510 AT4G13510 AMT1;1 ATAMT1 ATAMT1;1 ammonium transporter 1;1 ARABIDOPSIS THALIANA AMMONIUM TRANSPORT 1 T6G15.60 T6G15_60 WebThe NH 4 1 concentration needed to saturate AtAMT1;2 was much higher than for AtAMT1;1, which has a more than 10-fold higher affinity (Mayer and Ludewig, 2006; … h&m baberos

IJMS Free Full-Text Ectopic Expression of Sugarcane ScAMT1.1 …

WebNational Center for Biotechnology Information WebMotivation: Identifying regulatory modules is an important task in the exploratory analysis of gene expression time series data. Clustering algorithms are ofte WebApr 1, 2001 · Unlike AtNrt2.1, AtAmt1.1 expression is not stimulated in a nitrate reductase-deficient mutant after transfer to NO3- as sole N source, indicating that NO3- per se acts as a signal repressing transcription of AtAmt1.1. These results reveal two fundamentally different types of mechanism involved in the feedback regulation of root N acquisition ... hmb45 staining melanoma

Three polarly localized ammonium transporter 1 members are ...

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WebIn China, nitrogen (N) fertilizer is excessively used in sugarcane planting areas, while the nitrogen use efficiency (NUE) of sugarcane is relatively low. Mining and identifying the key genes in response to low N stress in sugarcane can provide useful gene elements and a theoretical basis for developing sugarcane varieties with high NUE. In our study, RNA … WebApr 1, 2001 · PDF We have compared the biochemical properties of two different Arabidopsis ammonium transporters, AtAMT1;1 and AtAMT1;2, expressed in yeast, … famzoo bbbWebJan 25, 2024 · CPK32 could interact with AtAMT1.1 and phosphorylate AtAMT1.1 at the non-conserved serine residue Ser450 in the CTR to enhance ammonium uptake in the natural environment (Qin et al. 2024). This study revealed a new mechanism underlying regulation of normal ammonium uptake. Taken together, the posttranslational regulation … hm babatka

"WebDec 4, 2024 · AtAMT1;1 was up-regulated under nitrogen deficiency (Shelden et al. 2001) and mediated ammonium influx (km = 34 µM), indicating that AtAMT1;1 is a high-affinity ammonium transporter (Wood et al. 2006). Disruption of AtAMT1;1 was found to result in ~ 70% ammonium absorption capacity retention in Arabidopis roots (Kaiser 2002). " - Atamt1 1

Atamt1 1

Frontiers Divergence of functions and expression patterns of …

WebDec 10, 2008 · Total RNA (20–30 μ g per lane) was separated by formaldehyde agarose gel electrophoresis, and RNA was transferred to a nylon membrane. DNA fragments carrying the ORFs of AtAMT1;1 or AtAMT1;4 were labeled with 32 P as probes for RNA hybridization. Preparation of membrane fractions and protein gel blot analysis. Total … WebNov 3, 2006 · AtAmt1.1 functions as a saturable high-affinity transporter with a K m of 65 μ m for methylamine, an ammonium analog. Using AtAmt1.1, two homologous cDNAs, …

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WebJan 1, 2024 · that AtAMT1;1 is a high-aﬃnity ammonium transporter (Wood e t al. 2006). Disruption of AtAMT1;1 was found to . result in ~ 70% ammonium absor ption capacity retention in . WebConstruction of an AtAmt1.1-Gal4 Driver System for LUC, Red-Shifted GFP, and GUS. We constructed a reporter system for nitrogen stress that fulfilled multiple criteria. LUC was …

WebEngineer C B, Kranz R G (2007) Reciprocal leaf and root expression of AtAmt1.1 and root architectural changes in response to nitrogen starvation. Plant Physiology 143:236–250 Gifford M L, Dean A, Gutierrez R A, Coruzzi G M, Birnbaum K D (2008) Cell-specific nitrogen responses mediate developmental plasticity. Proc. Natl. Acad. WebDec 15, 2006 · The atamt1;1-1 insertion line, rather than wild-type Arabidopsis, was used for transformation to circumvent an interaction of endogenously and ectopically expressed …

WebAtAMT1;1 this high anity is easily explained since plants are always competing for sucient ammonium uptake from the soil with other plants or microorganisms. High ammo-nium … WebMay 21, 2024 · AtAMT1;3 is a major contributor to high-affinity ammonium uptake in Arabidopsis roots. Using a stable electrophysiological recording strategy, we …

WebMay 21, 2024 · Figure 1. NH 4 + currents recorded with different recording strategies in oocytes. AtAMT1;3-expressing oocytes were subjected to 2 successive cycles of 2 perfusion solutions, the first solution being free from NH 4 + and the second one containing 1 mM NH 4 + (same solution background, pH 7.4). For each cycle, the oocyte was successively …

WebSep 1, 2024 · While AtAMT1–1 and 1–3 exhibited more similarity than AtAMT1–4, AtAMT1–4 was separated from all AMT1s in group B. In view of exon numbers, it was seen that the S1AMT1–2 gene contains only two exons, and this difference was observed in the phylogenetic tree, proposing that gene variations may cause this differentiation. hm babatka chlapciWebJan 4, 2024 · Switching to the expression of AtAMT1;1 driven by ProAtUBQ10 or of AtAMT1;1 mutated at two siRNA‐targeted hotspots reduced AtAMT1;1‐specific siRNAs and overcame S‐PTGS in roots. In roots of these lines, however, the steady‐state transcript levels of AtAMT1;1 still significantly decreased under conditions of N‐sufficiency … hm babatka dievcatkaWebAbstract. We have compared the biochemical properties of two different Arabidopsis ammonium transporters, AtAMT1;1 and AtAMT1;2, expressed in yeast, with the … hm babatka dievcata