Circrna pulldown probe

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August undefined, 2024

WebJul 19, 2024 · Circular RNAs (circRNAs), a recently identified new member of non-coding RNAs, are demonstrated to participate in diverse biological processes, such as … WebJul 19, 2016 · 环状RNA研究方法之RNA Pull-Down. 环状RNA（circular RNA，circRNA）是近来研究很热门的一种特殊的长链非编码RNA。. 研究发现，circRNA 在人体细胞中广泛 …

CircCD44 plays oncogenic roles in triple-negative breast cancer …

WebJan 1, 2015 · After incubation of LOC100506178 probes with streptavidin-coated magnetic beads, ... The RNA pull-down assay was adapted from previously published studies [27] or protocols [28]. Unedited and ... WebcircRNA Pull-down Assay. IntegrateRNA is an outstanding biotechnology company with years of experience in RNA-protein interaction analysis. We offer customized circRNA … how large is lisbon

Circ_CEA promotes the interaction between the p53 and cyclin …

WebThe Endogenous circRNA_0000429 pull-down by AGO2 was enriched in cells transfected with miRNA-1197 . To investigate the function of miRNA-1197 with circRNA_0000429, a circRNA-specific probe was used to pull down its interactive miRNA-1197s. circRNA_0000429 precipitation complex performed the enrichment of miRNA-1197 ( … http://www.circrna.com.cn/?p=4923 WebOct 22, 2024 · Circular RNAs (circRNAs) are a covalently enclosed endogenous biomolecules, which has tissue specificity and cell specificity in eukaryotes, and are produced after being regulated by specific cis... how large is las vegas

环状RNA研究方法之RNA Pull-Down

WebAug 31, 2024 · The next day, an M-280 Dynabeads probe-circRNA mixture was washed and incubated with 200 uL of lysis buffer. Finally, TRIzol was added to the mixture for RNA extraction and detection. The circRNF13 probe and primers used are listed in Table S1. Dual-luciferase reporter assay WebApr 11, 2024 · Nuclear-cytoplasmic fractionation and FISH were used to identify the localization of circRNA and miRNA. Pull-down, RIP, and luciferase activity assays were performed to show the biding ability of circRNA, miRNA and target proteins. ... Target Prove is BaseScope Probe-BA-Hs-PALM-E7E2-circRNA (Advanced Cell Diagnostics China, … how large is lithuania square milesWebFeb 4, 2024 · CircRNA has emerged as a new non-coding RNA that plays crucial roles in tumour initiation and development. ‘MiRNA sponge’ is the most reported role played by circRNAs in many tumours. how large is lexington ky

"WebThe RNA pull-down method can specifically and efficiently enrich circRNAs and their interacting partners. In order to further characterize the functions and working … " - Circrna pulldown probe

Circrna pulldown probe

Circular RNA circUBE2J2 acts as the sponge of microRNA-370-5P …

WebFeb 14, 2024 · CircRNAs are a class of covalently closed cyclic RNA molecules, most of which are resistant to RNase R digestion and are generally produced by the back-splicing of linear pre-mRNA. 11, 12 CircRNAs... WebFeb 16, 2024 · The formation of the circFoxo3-p21-CDK2 ternary complex arrests the function of CDK2, which is normally required for cell cycle progression. Several complimentary approaches, including circRNA pulldown with targeted probes, siRNA, and RNase A protection assays, were used to validate this ternary complex (Du et al., 2016).

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WebJan 21, 2024 · To determine the possible mechanisms underlying the effects of circRNA 100146 on general transcription, we designed specific circRNA 100146 protein-pull-down probe and isolated the interacting proteins in H460 cells (Additional file 4: Figure S3b). Protein mass spectrometry facilitated the identification of 657 proteins in total. WebSep 9, 2024 · RNA pull down and mass spectrometry Biotin-labeled oligonucleotide probes targeting junction sites of circRNAs were synthesized by SunBio Biomedical Technology Co., Ltd (Shanghai, China)....

WebCircRNA pull-down assay. A biotin-labeled circPTPRA probe was designed and synthesized by RiboBio Co., Ltd. The RNA pull-down assay was performed according to the instruction of the Pierce™ Magnetic RNA–Protein Pull-Down Kit (Thermo Scientific). The probe sequences used for the RNA pull-down assay are summarized in Additional … WebJan 8, 2024 · Circular RNA (CircRNA), a novel type of non-coding RNA, is formed by covalently closed loop structures without free terminal ends ( Chen, 2024 ). Due to their …

Web(c-e) RNA-Pulldown was performed in PCa cells using hsa_-circ_0063329 and oligo probes. (f) Luciferase reporter assay was used to determine the effects of four predicted miRnas on the luciferase ... WebOct 21, 2024 · Probe sequences were listed in the Major Resources Table in the Data Supplement. Statistical Analysis. All statistical analyses were performed using the GraphPad Prism software, version 8. ... . circRNA pull-down assay using biotin-labeled circSOD2 BSJ RNA probe followed by RT-qPCR identified 3 miRNA candidates that potentially bind …

WebAug 21, 2024 · The RNA pull-down method can specifically and efficiently enrich circRNAs and their interacting partners. In order to further characterize the functions and working …

WebSep 9, 2024 · RNA pull down and mass spectrometry Biotin-labeled oligonucleotide probes targeting junction sites of circRNAs were synthesized by SunBio Biomedical Technology … how large is little caesars pizzaWebJan 3, 2024 · circCDYL2 promotes trastuzumab resistance via sustaining HER2 downstream signaling in breast cancer - PMC Back to Top Skip to main content An official website of the United States government Here's how you know The .gov means it’s official. Federal government websites often end in .gov or .mil. how large is lincolnshireWebJun 2, 2024 · A novel and highly specific circRNA-pulldown followed by mass spectrometry analysis identified 94 circFOXK2-interacting proteins, which were involved in cell adhesion, mRNA splicing, and structural molecule activity. Of these, circFOKX2 interactions with YBX1 and hnRNPK enhanced expression of oncogenes NUF2 and PDXK. how large is londonWebJan 15, 2024 · Biotin-coupled miRNA and circRNA pull-down assays were performed as described previously ... In order to investigate whether miR-21-5p could bind to circRNA-ACAP2, we used a circRNA-ACAP2-specific probe to pull down its associated miRNAs. qRT-PCR results showed that miR-21-5p was greatly enriched in the circRNA-ACAP2 … how large is malta in milesWebFeb 28, 2024 · CircRNA pulldown. The specific steps of circRNA pulldown are described in our previous study . ... Cy3-labelled probe against circMUC16 (5’CY3-TCTTTCTCGGGCCTGAGCAAGGTCAGT- 3’CY3) and FITC-labeled probe against miR-199a were obtained from Genepharma. The FISH assay was performed following the … how large is little rock arkansasWeb新一代circRNA pull-down技术路线. 本项技术在吉赛生物circRNA过表达载体技术基础上，将RNA标签体系引入circRNA中，实现在细胞内表达带有特异性标签的circRNA；通过RNA标签与捕获蛋白的高度特异且稳定的相 … how large is loch ness in northern scotlandWebSep 1, 2024 · To pull down the miRNA by circRNA, biotinylated-circSMARCC1 probe (5′-CATCTTCCTCATCACAGCAC-3′) was synthesized by RiboBio (Guangzhou, China), and the oligo probe (5′-TATGTTGTTGATTTGCTGGC-3′) was used as a control. CircRNA pulldown assay was carried out using PierceTM Magnetic RNA-Protein Pull-Down Kit … how large is mainland france