Ea. hy926 cells

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August undefined, 2024

WebPurpose: The aim of the study was to evaluate the effect of piperlongumine (2 and 4 µM) on endothelial EA.hy926 and lung adenocarcinoma A549 cells with regulated expression of … WebJun 27, 2013 · EA.hy 926 is a permanent cell line derived by fusing human umbilical vein endothelial cells with cell line A549 [ 23 ]. The culture was maintained according to the protocol provided by the distributor of the cell line …

The effect of piperlongumine on endothelial and lung …

WebJul 27, 2024 · Human umbilical vein endothelial cells (HUVECs, Ea.hy926) were used to observe the cells adhesion to the samples. Firstly, the sterilized samples were put into 24-well culture plates, then the endothelial cells were seeded on the samples under a concentration of 2 × 10 4 cells/mL. After a 24 h incubation period, the samples were … WebApr 7, 2024 · EA.hy926 cells were cultured in 96-well plates and were serum-starved for 12 h in DMEM with 0.2% FBS before the vehicle, ET-1, or plant extract was added for 24 h. The Annexin V FITC Assay Kit (Cayman Chemical) was used and analyzed using Spectra Max Gemini EM at 485/535 nm per manufacturer protocols to determine the percentage of … simple python3 http server

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WebTo cite this cell line use: EA.hy 926 (RRID:CVCL_3901) Comments Group: Space-flown cell line (cellonaut). Characteristics: Produced by the fusion of A549/8 with human umbilical vein endothelial cells. Doubling time: 12 hours (PubMed= 6407019 ). Omics: Transcriptome analysis by microarray. WebEA.hy926 cells were treated with these gene complexes in the culture solution. EA.hy926 cells were treated with pEGFP-ZNF580 as a negative control group, and cells were treated with PEI 10 kDa/pDNA as a positive control group. The expression of GFP in transfected cells was observed under an inverted fluorescent microscope. WebJun 21, 2024 · Mechanistically, DOX-treated EA.hy926 cells and HUVECs demonstrated differential expression levels of the BCL-2 family proteins. In conclusion, both EA.hy926 … ray black hanwha

EA.hy926 Cells and HUVECs Share Similar Senescence Phenotypes but

Differential response to acute low dose radiation in …

WebEa.hy926 cells were grown under 2D or 3D conditions for 3 days and stained with antibodies to ICAM-1 ( A – C ) or VEGFR-2 ( D – F ). Cultures were added with TNF ( B , E ) or VEGF ( C , F ) to a concentration of 25 ng/mL within the last 5 hours. The solid gray peak is the autofluorescence of unstained cells; the red line is the ... WebA permanent human cell line (EA.hy926) preserves the characteristics of endothelin converting enzyme from primary human umbilical vein endothelial cells. Life Sci. … simple python code for adding two numbersWebOct 31, 2024 · EA.hy926 endothelial cells were seeded into a 96-well plate at the density of 3 × 104 cells per well with various concentrations of leptin (0, 0.1, 1, 5, 10, or 50 ng/ml). … simple pythagorean theorem calculator

"WebPurpose: The low dose radiation response of primary human umbilical vein endothelial cells (HUVEC) and its immortalized derivative, the EA.hy926 cell line, was evaluated and … " - Ea. hy926 cells

Ea. hy926 cells

EAhy 926 cell - European Bioinformatics Institute

WebMay 10, 2024 · Our results indicate that 1) EA.hy926 cells internalize B-ELNs in a dose-dependent manner; 2) pretreatment with B-ELNs counters TNF-α-induced reactive oxygen species (ROS) generation and loss of cell viability and modulates the differential expression of 29 genes (fold change > 1.5) induced by TNF-α compared to control; 3) pathway … WebFigure 1 The effect of piperlongumine on the viability of EA.hy926 (A) and A549 (B) cells. Notes: The cells were treated with different concentrations of piperlongumine (1–8 μM) for 24 hours and cell viability was assessed by MTT colorimetric assay. Data are expressed as a percentage of the untreated cells (control). Statistically significant difference at P<0.05 …

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WebJun 3, 2016 · (E) EA.hy926 cells were stimulated with individual human recombinant histone (20 μg/mL H1, H2A/H2B, H3.3, H4) for 4 h, and the expression of surface TF antigens was determined by flow cytometry. All data are presented as mean ± SEM. The data were combined data from 3 or more different experiments. WebMay 15, 2024 · EA.hy926 endothelial cells were seeded at a density of 9000 cells/cm 2 on non-coated (Panel A), Matrigel-coated (Panel B) or collagen-coated (Panel C) 96 well …

WebMar 1, 1996 · EA.hy926 cells were found to be more responsive than HUVECs to thrombin, as a lower concentration of the agonist was necessary to induce maximum monolayer permeability. Responses of HUVECs and EA.hy926 cells to thrombin were consistent with a pathway involving the thrombin receptor. A thrombin mutant in which the active-site … WebMay 26, 2024 · EA.hy926 cells were seeded in 96-well plates at a concentration of 1 × 10 5 cells/mL and incubated at 37 °C under 5% CO 2 humidified conditions for 24 h. Once the plated cells reached 100% confluence, 10 μL of DAF-FM-DA (10 mM) was added and the culture was incubated for 30 min. The cell culture medium was then removed and …

WebEA.hy926 endothelial cells were localized at the bottom of the cell insert and the alveolar type II A549 cell on the upper side (Figure 3a). Cells that resemble the round morphology of THP-1 were seen on top of the epithelial A549 cells ( Figure 3 b), which were recognized by the presence of small microvilli (arrows) and lamellar bodies (LM). WebJul 21, 2015 · EA.hy926 cells were transduced with lentivirus-based shRNA targeting NS, STING (B), or cGAS (C). Cells were then infected with KSHV (30 DNA genome copies per cell) for 8 h before IFN-β mRNA was measured by real-time qPCR. The relative amount of IFN-β mRNA was normalized to the 18S ribosomal RNA level in each sample, and the …

WebThe EAhy 926 cell is a hybridoma line derived from human endothelium and A549/8 cells. They display stable endothelial characteristics and may provide an indication of how endothelial cells respond to photodynamic therapy. Synonyms: EA.hy926 cell Tree view Term mappings - Graph view Reset tree Show all siblings experimental factor material …

WebMar 31, 2024 · EA.hy926 cells, transfected with striatin siRNA or controls (untransfected), were treated with estrogen (5 × 10−8 mol/L), and mRNA levels of PTGIS (A) and PTGS1 (B) were determined. Results show no significant difference in the genomic response after 5 hours of estrogen stimulation in the transfected and control cells (*, P < .05 vs vehicle). ray black matte ban eyeglassesWebThis product is no longer available on Biocompare. Product Specs. Item EA.hy926. Company ATCC. Catalog Number CRL-2922. simple pythagoras theoremWebMay 15, 2024 · EA.hy926 endothelial cells were seeded at 9000 cells/cm 2 on 96 well plates pre-coated with Matrigel (5 μL Matrigel mixed in 95 μL DMEM) or without Matrigel (Panel A) or Collagen Type I dissolved in acetic acid at concentrations of 6 and 10 μg/cm 2 (Panel B) and grown for up to 10 days without changing the media. Cell number was … simple python adventure gameWebDec 18, 2024 · Before You Begin EA.hy926 Cell Culture. Timing: 2 days. 1. We have generated EA.hy926 cells (ATCC) stably expressing pLenti-blasticidin-GFP or pLenti … simple python 3 programsWebMar 15, 2024 · EA.hy926 cells express very little RNA for caspase-5 and inflammasome sensors, NLRP3 and NLRC4, that are essential for caspase-1 activation . We stimulated … simple pythagoras worksheetWebJan 10, 2024 · EA.hy926 is a human umbilical vein cell fusion line that is widely used in vascular-related research. LPS causes inflammation in the endangium, which damages the integrity of endothelial cells. simple python benchmarkWebOct 25, 2024 · Senescent EA.hy926 cells express a β-gal, which was detected using a Beta Galactosidase (β-gal) Activity Assay kit (BioVison, Milpitas, CA, USA), according to the manufacturer's instructions, based on the formation of a local blue precipitate upon enzyme-mediated cleavage. simple pythagorean theorem