WebI think you can use both of them at the same time. Because DAPI is a DNA minor groove agent and PI is an intercalator. This means they are going to bind different parts of the same DNA. WebJan 1, 2011 · A number of fluorescent stains are available that label DNA and allow easy visualization of the nucleus in interphase cells and chromosomes in mitotic cells, including Hoechst, 4',6-diamidino-2-phenylindole (DAPI), ethidium bromide, propidium iodide, and acridine orange.

DAPI for nucleic acid staining Sigma-Aldrich

Web1. Wash the cells 1–3 times in PBS as needed. 2. Add sufficient 300 nM DAPI stain solution to cover the cells. 3. Incubate for 1–5 minutes, protected from light. 4. Remove the stain solution. 5. Wash the cells 2–3 times in PBS. 6. Image the cells. Spectral information and storage Protocol tips WebMar 1, 2015 · CTCs in 3.75 mL of blood were depleted for CD45 positive cells with anti-CD45 antibodies and identified by combining CK, CD45, DAPI and CEP8 in 61 cases including 22 pancreatic cancers, 3 borderline pancreatic solid pseudopapillary tumors, 6 pancreatic benign tumors, and 30 healthy individuals. bus 55 oahu time table

Patterns of circulating tumor cells identified by CEP8, CK and ... - PubMed

WebDAPI was first synthesised in 1971 in the laboratory of Otto Dann as part of a search for drugs to treat trypanosomiasis. Although it was unsuccessful as a drug, further investigation indicated it bound strongly to DNA and … WebDOWNLOADS Most Popular Insights An evolving model The lessons of Ecosystem 1.0 Lesson 1: Go deep or go home Lesson 2: Move strategically, not conveniently Lesson 3: Partner with vision Lesson 4: Clear the path to impact The principles of Ecosystem 2.0 … WebMar 14, 2024 · However, severe inflammation can also produce similar symptoms in cells. In this study, normal, inflammatory, and carcinoma hepatic cells were stained with 4',6-diamidino-2-phenylindole (DAPI) and investigated by hyperspectral microscopy. DAPI is … ham with white sauce