Mitotracker orange 原理
http://www.kenkyuu2.net/cgi-biotech2012/biotechforum.cgi?mode=view;Code=4457 Web当细胞长至所需丰度,吸除培养液,加入37℃预热的 MitoTracker® Green FM染色工作液。 在所使用细胞正常培养条件下孵育15-45 min(最佳孵育时间需优化)。 染色结束后,使 …
Mitotracker orange 原理
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Web22 jun. 2011 · Systematic colocalization errors between acridine orange and EGFP in astrocyte vesicular organelles: F. Nadrigny, et al.; Biophys. J. 93, ... Chloromethyl-X-rosamine (MitoTracker Red) photosensitises mitochondria and induces apoptosis in intact human cells: T. Minamikawa, et al.; J. Cell. Science 112, 2419 (1999), Abstract; WebMitoTracker探针是一种小的(<1kDa)细胞渗透性线粒体选择性染料,含有巯基反应性氯甲基基序,在固定后仍然维持在线粒体内。 因为探针与线粒体硫醇形成共价键,所以它们 …
WebMitoTracker™ Orange CM-H2TMRos 是还原性、无荧光版本的 MitoTracker Orange (M-7510),氧化后发荧光。 该染料还可染色活细胞中的线粒体并随膜电位积聚。 醛固定后 …
WebMitoTracker® Deep Red FM是一种细胞渗透型的carbocyanine-based的远红外红色荧光探针(Ex=644 nm,Em=665nm),包含标记线粒体的弱巯基反应性的氯甲基官能团,只需简单孵育细胞,即可被动运输穿过细胞膜并直接聚集在活性线粒体上。一旦线粒体被染色后,还能根据后续实验的需求进行固定(醛类固定剂如甲醛 ... WebMitoTracker® Red FM is a far red-fluorescent dye (abs/em ∼581/644 nm) that stains mitochondria in live cells and its accumulation is dependent upon membrane potential. For Research Use Only. Not for use in diagnostic …
WebMitoTracker® Orange CMTMRosは遠赤外蛍光色素と互換性があります。 図 2.MitoTracker® Red 色素を使用した、アポトーシスにおけるミトコンドリア膜電位の …
Web9 mrt. 2015 · LysoTracker® and LysoSensor™ Probes 2 LysoSensor™ pH Indicators For researchers studying the dynamic aspects of lysosome biogenesis and function in live cells, we offer LysoSensor™ probes—fluorescent pH indicators that partition into acidic organelles. The LysoSensor™ dyes are acidotropic probes that appear to accumulate in … hotels near arugam bay beachWeb2 mrt. 2024 · You can use MitoTracker with MFN-1, DRP-1 or other mitochondrial dynamics marker to asses mitochondrial fission or fusion. You can check the mitochondrial dyes in detail here.... hotels near asb tennis centreWebPopular answers (1) 20th Apr, 2016. Jitendra Singh. National Institute of Pharmaceutical Education and Research. MitoTracker passively diffuse across the plasma membrane and accumulate in active ... hotels near asakusa train stationWeb2 sep. 2008 · mito trackerでミトコンドリアが染色できるそうなのですが本を読んでも原理がわかりません。 mito trackerがどのような物質でミトコンドリアの中でどのように … hotels near arvin high schoolWebMito-Tracker Green,也称MitoTracker Green,是一种线粒体(mitochondria)绿色荧光探针,可以用于活细胞线粒体特异性荧光染色。 Mito-Tracker Green为采用Molecular Probes公司的carbocyanine进行了荧光标记的一种Mito-Tracker, 也称Benzoxazolium, 2-[3-[5,6-dichloro-1,3-bis[[4-(chloromethyl)phenyl]methyl]-1,3-dihydro-2H-benzimidazol-2-ylidene] … lily bloom customer serviceWebミトコンドリアは我々ヒトを始めとするほとんどの真核生物の細胞内に存在する細胞小器官の1つです。. 数ある細胞小器官のなかでも特にエネルギー産生(アデノシン三リン … lily bloom christmas pursesWeb1 sep. 2015 · In contrast, MitoTracker Red CMXRos, tetramethylrhodamine methyl ester, and 10-N-nonyl acridine orange label mitochondria in a manner dependent on the membrane potential, thus giving an indication of mitochondrial stress. Using MitoSOX Red, it is also possible to analyze the production of the mitochondrial superoxide anion. lily bloom crossbody handbags